scott gradia (Addgene inc)
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scott gradia
Scott Gradia, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scott+gradia/pET+His6+TEV+LIC+cloning+vector+(1B)+(Plasmid+%2329653)/pmc13044922-77-9-11
Average 94 stars, based on 119 article reviews
Scott Gradia, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scott+gradia/pET+His6+TEV+LIC+cloning+vector+(1B)+(Plasmid+%2329653)/pmc13044922-77-9-11
Average 94 stars, based on 119 article reviews
scott gradia - by Bioz Stars,
2026-10
94/100 stars
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Cloning:Article Title: Molecular structure and nickel-binding capacity of Proteus mirabilis UreE Article Snippet: To generate the Pm UreE expression plasmid (pETHis6TEVLIC_UreE), the P. mirabilis ATCC 12453 ureE open reading frame (486 bp) and the kanamycin-resistant pETHis6TEVLIC plasmid backbone were amplified separately by PCR using Q5 High-Fidelity 2× Master Mix (New England Biolabs; primers are listed in Supplementary Table S1). .. The pETHis6TEVLIC cloning vector (1B) was a gift from Article Title: Atg23 Interacts With Both the N- and C-termini of Atg9 Via a Hydrophobic Binding Pocket Article Snippet: GST-Atg9 12-24 was generated by insertional mutagenesis into the pET His6 GST TEV LIC cloning vector (1G), which was a gift from Scott Gradia (Addgene plasmid # 29655) using Q5 site directed mutagenesis. .. 6xHis-Atg9-1-255-TEV-MBP constructs was generated by Gibson cloning between the His-tag and MBP of the pET His6 MBP TEV LIC cloning vector (1M), which was a gift from Article Title: VRK1 co-delivery mitigates DNA clustering by BAF in TFAMoplex transfection Article Snippet: .. All pET vectors used in this study are derived from the pET His6 TEV LIC cloning vector (1B), developed by Article Title: Atg23 Interacts With Both the N- and C-termini of Atg9 Via a Hydrophobic Binding Pocket Article Snippet: .. GST-Atg9 12-24 was generated by insertional mutagenesis into the pET His6 GST TEV LIC cloning vector (1G), which was a gift from Article Title: Molecular structure and nickel-binding capacity of
<i>Proteus mirabilis</i>
UreE Article Snippet: To generate the PmUreE expression plasmid (pETHis6TEVLIC_UreE), the P. mirabilis ATCC 12453 ureE open reading frame (486 bp) and the kanamycin-resistant pETHis6TEVLIC plasmid backbone were amplified separately by PCR using Q5 High-Fidelity 2� Master Mix (New England Biolabs; primers are listed in Supplementary Table S1). .. The pETHis6TEVLIC cloning vector (1B) was a gift from Article Title: Molecular structure and nickel binding capacity of Proteus mirabilis UreE Article Snippet: To generate the Pm UreE expression plasmid (pETHis6TEVLIC_UreE), the P. mirabilis ATCC 12453 ureE open reading frame (486 bp) and the kanamycin-resistant pETHis6TEVLIC plasmid backbone were amplified separately by PCR using Q5 High-Fidelity 2× Master Mix (New England Biolabs) (primers listed in ). .. The pETHis6TEVLIC cloning vector (1B) was a gift from Plasmid Preparation:Article Title: Molecular structure and nickel-binding capacity of Proteus mirabilis UreE Article Snippet: To generate the Pm UreE expression plasmid (pETHis6TEVLIC_UreE), the P. mirabilis ATCC 12453 ureE open reading frame (486 bp) and the kanamycin-resistant pETHis6TEVLIC plasmid backbone were amplified separately by PCR using Q5 High-Fidelity 2× Master Mix (New England Biolabs; primers are listed in Supplementary Table S1). .. The pETHis6TEVLIC cloning vector (1B) was a gift from Article Title: Atg23 Interacts With Both the N- and C-termini of Atg9 Via a Hydrophobic Binding Pocket Article Snippet: GST-Atg9 12-24 was generated by insertional mutagenesis into the pET His6 GST TEV LIC cloning vector (1G), which was a gift from Scott Gradia (Addgene plasmid # 29655) using Q5 site directed mutagenesis. .. 6xHis-Atg9-1-255-TEV-MBP constructs was generated by Gibson cloning between the His-tag and MBP of the pET His6 MBP TEV LIC cloning vector (1M), which was a gift from Article Title: VRK1 co-delivery mitigates DNA clustering by BAF in TFAMoplex transfection Article Snippet: .. All pET vectors used in this study are derived from the pET His6 TEV LIC cloning vector (1B), developed by Article Title: Crystallographic data for Pyrococcus furiosus dolichylphosphate mannose synthase suggest that the enzyme could flip its glycolipid product. Article Snippet: .. The 2GFP-T plasmid was a gift from Article Title: Atg23 Interacts With Both the N- and C-termini of Atg9 Via a Hydrophobic Binding Pocket Article Snippet: .. GST-Atg9 12-24 was generated by insertional mutagenesis into the pET His6 GST TEV LIC cloning vector (1G), which was a gift from Article Title: Molecular structure and nickel-binding capacity of
<i>Proteus mirabilis</i>
UreE Article Snippet: To generate the PmUreE expression plasmid (pETHis6TEVLIC_UreE), the P. mirabilis ATCC 12453 ureE open reading frame (486 bp) and the kanamycin-resistant pETHis6TEVLIC plasmid backbone were amplified separately by PCR using Q5 High-Fidelity 2� Master Mix (New England Biolabs; primers are listed in Supplementary Table S1). .. The pETHis6TEVLIC cloning vector (1B) was a gift from Article Title: Molecular structure and nickel binding capacity of Proteus mirabilis UreE Article Snippet: To generate the Pm UreE expression plasmid (pETHis6TEVLIC_UreE), the P. mirabilis ATCC 12453 ureE open reading frame (486 bp) and the kanamycin-resistant pETHis6TEVLIC plasmid backbone were amplified separately by PCR using Q5 High-Fidelity 2× Master Mix (New England Biolabs) (primers listed in ). .. The pETHis6TEVLIC cloning vector (1B) was a gift from Article Title: PXL: a Nucleic Acid–Binding Module of Promyelocytic Leukemia Protein Article Snippet: .. The vector was a gift from Construct:Article Title: Atg23 Interacts With Both the N- and C-termini of Atg9 Via a Hydrophobic Binding Pocket Article Snippet: GST-Atg9 12-24 was generated by insertional mutagenesis into the pET His6 GST TEV LIC cloning vector (1G), which was a gift from Scott Gradia (Addgene plasmid # 29655) using Q5 site directed mutagenesis. .. 6xHis-Atg9-1-255-TEV-MBP constructs was generated by Gibson cloning between the His-tag and MBP of the pET His6 MBP TEV LIC cloning vector (1M), which was a gift from Generated:Article Title: Atg23 Interacts With Both the N- and C-termini of Atg9 Via a Hydrophobic Binding Pocket Article Snippet: GST-Atg9 12-24 was generated by insertional mutagenesis into the pET His6 GST TEV LIC cloning vector (1G), which was a gift from Scott Gradia (Addgene plasmid # 29655) using Q5 site directed mutagenesis. .. 6xHis-Atg9-1-255-TEV-MBP constructs was generated by Gibson cloning between the His-tag and MBP of the pET His6 MBP TEV LIC cloning vector (1M), which was a gift from Article Title: Atg23 Interacts With Both the N- and C-termini of Atg9 Via a Hydrophobic Binding Pocket Article Snippet: .. GST-Atg9 12-24 was generated by insertional mutagenesis into the pET His6 GST TEV LIC cloning vector (1G), which was a gift from Mutagenesis:Article Title: Atg23 Interacts With Both the N- and C-termini of Atg9 Via a Hydrophobic Binding Pocket Article Snippet: GST-Atg9 12-24 was generated by insertional mutagenesis into the pET His6 GST TEV LIC cloning vector (1G), which was a gift from Scott Gradia (Addgene plasmid # 29655) using Q5 site directed mutagenesis. .. 6xHis-Atg9-1-255-TEV-MBP constructs was generated by Gibson cloning between the His-tag and MBP of the pET His6 MBP TEV LIC cloning vector (1M), which was a gift from Article Title: Atg23 Interacts With Both the N- and C-termini of Atg9 Via a Hydrophobic Binding Pocket Article Snippet: .. GST-Atg9 12-24 was generated by insertional mutagenesis into the pET His6 GST TEV LIC cloning vector (1G), which was a gift from Derivative Assay:Article Title: VRK1 co-delivery mitigates DNA clustering by BAF in TFAMoplex transfection Article Snippet: .. All pET vectors used in this study are derived from the pET His6 TEV LIC cloning vector (1B), developed by |